Information-enriched characterization of cell populations. Morphological data from images can insert to your richness of flow cytometry data. Such as, the determine displays an or else typical apoptosis assay working with Annexin V and PI, introducing cell imaging to characterize cells in Each individual populace to expose morphologically unique attributes. These insights couldn't are obtained from multiplex staining by yourself.
As samples are acquired to the Attune CytPix Flow Cytometer, the superior-pace brightfield digital camera captures and retailers illustrations or photos of detected situations in a rate approximately 6,000 images for every 2nd, dependant upon the flow price and impression dimensions. For higher overall flexibility, Attune Cytometric Software package lets you change the graphic seize frequency as required and choose a certain gate for image capture, if necessary.
Mix acoustic concentrating engineering with superior-parameter Assessment for versatile spectral unmixing and standard workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating that has a high-pace brightfield digicam for simultaneous substantial throughput flow cytometry and large resolution brightfield imaging.
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On top of that, it is illuminating to determine how many doublets slip by way of standard singlet gates, and good to validate the position of unpredicted double-optimistic activities in my Examination."
Pair your Attune flow cytometer with the autosampler for amplified workflow performance, and increase robotic integration for maximum throughput.
Our researchers will style and design panels in your case as often times you need—totally free, no products invest in desired.
Early signs of this contamination experienced initially been dismissed as particles. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also enables you to use morphological capabilities to discover attention-grabbing subpopulations that could not be clear from flow cytometry data alone.
Buyers can look at event pictures in genuine-time to substantiate sample good quality, enhance protocols, and history the morphology of each and every cell inhabitants discovered for Examination. Imaging seize also Gains from acoustic concentrating, which helps to place the cells making sure that a pointy, centered image is acquired.
AI-pushed automatic image Evaluation Together with the Attune Cytometric Application translates function functions into distinct morphology parameters which might be coupled with standard fluorescence and scatter parameters to expose extremely reproducible inhabitants variations and one of a kind subsets.
We Beforehand shown the strength of imaging Car or truck-T/Ramos mobile interactions. Permit’s check out just the populace of greatest desire, the double favourable events, To find out more. We will now use extended morphological parameters (in this article, circularity and skewness of intensity) to more study the attributes of those populations and refine gating on these events, growing details robustness.
Constructed-in dimensionality reduction applications elevated resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to verify envisioned populace resolution in lysed whole human blood. User can check out situations from Each individual cluster for visual affirmation of cell phenotypes.
No wash/no lyse click here leukocyte Assessment with imaging. Cells had been obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Expert UX/UI enables consumers to prepare AI styles for expanded apps. (A) The “Educate Design” feature delivers annotation applications and genuine-time product schooling accuracy.
At the center of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating know-how, which makes use of sound waves to meticulously align cells throughout the flow cell. This modern approach surpasses common hydrodynamic strategies, resulting in more quickly processing speeds, Excellent details excellent, and enhanced sample throughput.
The Attune Xenith cytometer is often a high-parameter spectral analyzer, providing each spectral unmixing and traditional flow capabilities for in depth and adaptable mobile Investigation.
The Attune CytPix Flow Cytometer allows end users to visualize samples during acquisition, confirm the accuracy of gating approaches, and assistance effects with reputable documentation for publication needs.
What appeared to be a homogeneous inhabitants is actually much more varied—an insight that ought to be regarded as when interpreting final results.